Lipid analysis

نویسندگان

  • Peter D. Walsh
  • Kate A. Abernethy
  • Magdalena Bermejo
  • Rene Beyers
  • Pauwel De Wachter
  • Marc Ella Akou
  • Bas Huijbregts
  • Kamdem Toham
  • Christian Mbina
  • Yves Mihindou
  • Sosthène Ndong
  • Ernestine Ntsame Effa
  • Malcolm P. Starkey
  • Paul Telfer
  • Marc Thibault
  • Caroline E. G. Tutin
  • Lee J. T. White
  • David S. Wilkie
چکیده

Particulate organic matter for lipid analyses was collected from specific water depths by in situ filtration of large volumes (,1,000 l) of water through 292-mm diameter precombusted (at 450 8C) glass fibre filters (GFF; nominal pore size 0.7 mm) with in situ pumps. Because filtration through 0.7-mm pore-size filters could lead to an undersampling of anammox cells, the calculated ladderane lipid concentrations are minimum values. The GFF were extracted for 24 h in a Soxhlet apparatus to obtain the total lipid extracts. Aliquots of the total extracts were saponified after addition of an internal standard and separated into fatty acid and neutral lipid fractions. The fatty acid fractions were methylated and the neutral fractions were silylated and analysed by gas chromatography– mass spectrometry for the identification and quantification of ladderane lipids. Repeated concentration measurements were within ^10%.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Effects of Dill Extract on Blood Lipid Levels (TC, TG, LDL and HDL): A Systematic Review and Meta-Analysis

Background and Objective: In recent years,there has been a growing trend  towards the use of herbal medicine in the treatment and prevention of diseases. Blood lipid-lowering drugs have many side effects. On the other hand, various studies have reported the effect of dill (Anethum graveolens) on the reduction of blood lipids in different ways. This study aimed to evaluate the effect of dill on ...

متن کامل

Despite Affordability, Low Utilization of Lipid Lowering Drugs in Iran: Trend Analysis and Benchmarking 2005-2016

Dyslipidemia is responsible for great mortality and morbidity each year. Little data are available on the availability and affordability of Dyslipidemia medications in low and middle incomes countries. In a retrospective time-series study, we examined the utilization pattern and affordability of lipid-lowering medications in Iran as a lower middle-income country. We initially calculated the def...

متن کامل

Despite Affordability, Low Utilization of Lipid Lowering Drugs in Iran: Trend Analysis and Benchmarking 2005-2016

Dyslipidemia is responsible for great mortality and morbidity each year. Little data are available on the availability and affordability of Dyslipidemia medications in low and middle incomes countries. In a retrospective time-series study, we examined the utilization pattern and affordability of lipid-lowering medications in Iran as a lower middle-income country. We initially calculated the def...

متن کامل

An experimental analysis of the effects of dietary lipid sources and feeding ration on the reproductive performance, egg and larval quality of Nile tilapia, Oreochromis niloticus (L.)

This study investigates the effects of dietary lipid sources on the growth and reproductive performance of Nile tilapia over three consecutive spawnings. Fish were reared using three experimental diets, with the goal of replacing dietary fish oil with palm oil. Three experimental diets and a commercial   trout diet was used as the control. The effect of dietary lipid on the growth performance, ...

متن کامل

Characterization of biophysical properties of single chloride channel in rat brain mitochondrial inner membrane by channel incorporation into bilayer lipid membrane

Introduction: Recent studies have shown the presence of Cl- channels in heart and liver mitochondrial membranes. In this work, we have characterized the functional profile of a Cl- channel from rat brain mitochondria. Methods: After removing and homogenizing the rat brain, the supernatant was separately centrifuged in MSEdigitonin, H2O and Na2CO3 and mitochondrial inner membrane vesicles wer...

متن کامل

Comparison of the effect of three therapies on liver enzymes and lipid profiles in hypercholesterolemic rats

Background and Aims: The present study aimed to compare the effects of three therapeutic methods (including herbal, atorvastatin, and exercise) on liver enzymes and lipid profiles in hypercholesterolemic rats. Materials and Methods: A number of 32 male Wistar rats were randomly assigned to four groups (n=8), including hypercholesterolemia (Hc) control group, anvil extract at a dose of 100 mg/k...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2003