Lipid analysis
نویسندگان
چکیده
Particulate organic matter for lipid analyses was collected from specific water depths by in situ filtration of large volumes (,1,000 l) of water through 292-mm diameter precombusted (at 450 8C) glass fibre filters (GFF; nominal pore size 0.7 mm) with in situ pumps. Because filtration through 0.7-mm pore-size filters could lead to an undersampling of anammox cells, the calculated ladderane lipid concentrations are minimum values. The GFF were extracted for 24 h in a Soxhlet apparatus to obtain the total lipid extracts. Aliquots of the total extracts were saponified after addition of an internal standard and separated into fatty acid and neutral lipid fractions. The fatty acid fractions were methylated and the neutral fractions were silylated and analysed by gas chromatography– mass spectrometry for the identification and quantification of ladderane lipids. Repeated concentration measurements were within ^10%.
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